MAP1LC3A Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90822-50, BS90822-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9H492(Human) Q91VR7(Mouse) Q6XVN8(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC, IP,
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3β) and MAP-light chain 3 alpha (MAP-LC3α), both of which are mammalian homologs of yeast Apg8, are subunits that can associate with either MAP-1A or MAP-1B. While MAP-LC3β is essential for autophagy and is associated with autophagosome membranes after processing, MAP LC3α is involved in the formation of autophagosomal vacuoles and is localized to the intracytoplasmic membrane. MAP LC3α is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MAP1LC3A polyclonal antibody detects endogenous levels of MAP1LC3A protein.
Bioworld Molecular Weight: 14 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MAP1LC3A on mouse brain lysates using anti-MAP1LC3A antibody at 1/1,000 dilution., ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MAP1A/MAP1B LC3 A, MAP1LC3A, ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, MAP1 light chain 3 like protein 1, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, Map1lc3a, Microtubule associated proteins 1A/1B light chain 3, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A and 1B, light chain 3, Microtubule-associated proteins 1A/1B light chain 3A, MLP3A,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified