Product Description

MAP1LC3A Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9925M-50, BS9925M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9H492

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:2000
IHC/ICC/IF: 1:50-1:200
FC: 1:50-1:100

Background: Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of MAP1LC3A and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 14 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MAP1LC3A Rabbit mAb at 1:1000 dilution
Lane 1: SHG-44 whole cell lysate
Lane 2: The brain tissue lysate of Mouse
Lane 3: The liver tissue lysate of Mouse
Lane 4: The skeletal muscle lysate of Mouse, Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin., ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilisedwith 0.25% Triton *100/PBS.

Alternative Name: ATG8E;
Autophagy-related protein LC3 A;
Autophagy-related ubiquitin-like modifier LC3 A;
LC3;
LC3A;
MAP1 light chain 3 like protein 1;
MAP1 light chain 3-like protein 1;
MAP1A/1B light chain 3 A;
MAP1A/MAP1B LC3 A;
MAP1A/MAP1B light chain 3 A;
MAP1ALC3;
MAP1BLC3;
Map1lc3a;
Microtubule associated proteins 1A/1B light chain 3;
Microtubule-associated protein 1 light chain 3 alpha;
Microtubule-associated proteins 1A/1B light chain 3A;
MLP3A_HUMAN;

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified