MAP2K4 Polyclonal Antibody-BS78912

MAP2K4 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS78912-50, BS78912-100

Swiss-Prot: P45985

Host: Rabbit

MAP2K4 Polyclonal Antibody-BS78912

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB,1:1000 - 1:4000 | IP,1:50 - 1:100

Background: This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 44KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HeLa, using MAP2K4 antibody at 1:750 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug MAP2K4 antibody. Western blot was performed from the immunoprecipitate using MAP2K4 antibody at a dilution of 1:1000.

Alternative Name: MAP2K4; JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1

Immunogen: A synthetic peptide of human MAP2K4(NP_003001.1).

Modification: Unmodified

Conjugate: Unconjugated