MAP3K5 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A12458-20, A12458-100
Citations, Manuals and MSDS Available upon request.
Background: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1, 374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK) /stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Category: Polyclonal Antibodies
Applications: WB, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 155kDa
Observed Molecular Weight: 160kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 4217
SWISS: Q99683
Alternate Names: ASK1; MEKK5; MAPKKK5; MAP3K5
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Protein phosphorylation, Signal Transduction, Kinase, Serine threonine kinases, MAPK-JNK Signaling Pathway, Cell Biology Developmental Biology, Apoptosis, Cytoskeleton, Actins, Death Receptor Signaling Pathway, Immunology Inflammation, B Cell Receptor Signaling Pathway, Neuroscience, Neurodegenerative Diseases, Cardiovascular, Heart, Hypertrophy.
NCBI Alias: MAP3K5
Research Use Only