MASP2 Polyclonal Antibody-BS5789

MASP2 Polyclonal Antibody

Catalogue Numbers: BS5789-50, BS5789-100

Sizes: 50µl, 100µl

Alternative Name: Mannan-binding lectin serine protease 2; MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP-2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00187

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Mannose (or mannan)-binding lectin (MBL), also known as serum mannosebinding protein (MBP), initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL is a key component in immune response in that it can directly trigger neutralization of invading microorganisms by an Ab-independent mechanism. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation. In human, three types of MBL-associated serine proteases, MASP-1, MASP-2 and MASP-3, and a truncated form of MASP-2 (small MBL-associated protein; sMAP or MAp19) complex with MBL to activate the lectin pathway of the complement system. Activated MASPs subsequently cleave and activate downstream components of the complement pathway. MASP-3 is an alternatively spliced product from the MASP-1 gene and may function to inhibit MASP-2 by competing for MBL binding and inhibiting the activation of MBLassociated MASP-2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MASP2 polyclonal antibody detects endogenous levels of MASP2 protein.

Bioworld Molecular Weight: ~ 76 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MASP2 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 268-313 of Human MASP2.

Conjugate: Unconjugated

Modification: Unmodified