Product Description

MATK Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8326-50, BS8326-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P42679

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200

Background: The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 56kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of THP-1 cells, using MATK antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded rat kidney using MATK antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse kidney using MATK antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Alternative Name: MATK; CHK; CTK; HHYLTK; HYL; HYLTK; Lsk

Immunogen: Recombinant fusion protein of human MATK(NP_647611.1).

Conjugate: Unconjugated

Modification: Unmodified