Product Description

MBD3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90828-50, BS90828-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95983(Human) Q9Z2D8(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IF, IHC FC IP,

All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5′ cytosine followed by guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferase, which results in transcriptional repression of the methylated gene. Several proteins have been identified that associate with the methyl-CpG sites, and they include methyl-CpG binding protein-1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: MBD3 polyclonal antibody detects endogenous levels of MBD3 protein.

Bioworld Molecular Weight: 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MBD3 on U937 cell lysate using anti-MBD3 antibody at 1/500 dilution., ICC staining MBD3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MBD3, AI181826, AU019209, MBD 3, Mbd3, MBD3: methyl CpG binding domain protein 3, MBD3, Methyl CpG binding domain protein 3, Methyl CpG binding protein MBD3, Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified