Product Description

MBP Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66778-50, MB66778-100

Product: Mouse IgM. Supplied in crude ascites with 0.01% sodium azide.

Swiss-Prot: P02686

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB (1/1000 - 1/5000), IF/ICC (1/10 - 1/50)

Background: Myelin basic protein (MBP) is the major extrinsic membrane protein of central nervous system myelin. MBP phosphorylation at Threonine 125 is a complex regulatory process that modulates the contribution of MBP to the stability of the myelin sheath. Mitogen-activated protein kinases modulate MBP phosphorylation during myelinogenesis and in the demyelinating disease multiple
sclerosis. MBP phosphorylation is regulated by high-frequency stimulation but not low-frequency stimulation of the alveus, the myelinated output fibers of the hippocampus. It is proposed that during periods of increased neuronal activity, calcium activates axonal nitric oxide synthase, which generates the intercellular messengers nitric oxide and superoxide and regulates the phosphorylation state of MBP by MAPK.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of MBP protein.

Bioworld Molecular Weight: ~ 31 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MBP expression in ZR751 (A) whole cell lysates., Immunofluorescent analysis of MBP staining in NCIH460 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: Myelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein

Immunogen: Recombinant fusion protein of human MBP. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified