Product Description

MBP Polyclonal Antibody

Catalogue Numbers: BS62262-50, BS62262-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P02686

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: Myelin basic protein (MBP) is the major extrinsic membrane protein of central nervous system myelin. MBP phosphorylation at Threonine 125 is a complex regulatory process that modulates the contribution of MBP to the stability of the myelin sheath. Mitogen-activated protein kinases modulate MBP phosphorylation during myelinogenesis and in the demyelinating disease multiple sclerosis. MBP phosphorylation is regulated by high-frequency stimulation but not low-frequency stimulation of the alveus, the myelinated output fibers of the hippocampus. It is proposed that during periods of increased neuronal activity, calcium activates axonal nitric oxide synthase, which generates the intercellular messengers nitric oxide and superoxide and regulates the phosphorylation state of MBP by MAPK.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MBP polyclonal antibody detects endogenous levels of MBP protein.

Extra Notes: Western blot (WB) analysis of MBP polyclonal antibody at 1:500 dilution
LaneA:U-87MG whole cell lysate
LaneB:The Brain tissue lysate of Mouse
LaneC:The Brain tissue lysate of Rat

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Myelin basic protein; Myelin A1 protein; Myelin membrane encephalitogenic protein; MBP

Immunogen: Synthetic peptide, corresponding to Human MBP.

Conjugate: Unconjugated

Modification: Unmodified