Product Description

MBP-Tag Mouse Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB2012-50, MB2012-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: MBP is a usefμl affinity tag that can increase the expression level and solubility of the resμlting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagμlation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.

Category: Primary Antibody

Reactivity: Transfected

Host: Mouse

Applications: WB

Alternate Names: MBP

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide MBP-Tag.

Dilution: WB: 1:5000~1:10000

Purification: Affinity-chromatography

Specificity: MBP-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal MBP -tagged recombinant proteins.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only