Product Description

MBP tag Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65618-50, BS65618-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a usefμl affinity tag that can increase the expression level and solubility of the resμlting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagμlation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

Category: Primary Antibody

Reactivity: Escherichia Coli

Host: Rabbit

Applications: WB

Alternate Names: MBP-tag, MBP, MMBP, Maltose Binding Protein, Maltose binding protein tag, ECK4026, JW3994, Mal E, MalE, malJ, Maltodextrin binding protein, Maltose ABC transporter periplasmic protein, Maltose binding periplasmic protein, Periplasmic maltose binding protein, MALE_ECOLI.

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein:1-100/396

Dilution: WB=1:500-2000

Purification: affinity purified by Protein A

Specificity: MBP tag Polyclonal Antibody detects over-expressed or recombinant proteins containing the MBP epitope tag.

Molecular Weight: 70 kD

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P0AEX9

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Research Use Only