MBTPS2 Polyclonal Antibody
Catalogue Numbers: BS61210-50, BS61210-100
Sizes: 50µl, 100µl
Alternative Name: Membrane-bound transcription factor site-2 protease (EC:3.4.24.85); Endopeptidase S2P; Sterol regulatory element-binding proteins intramembrane protease; SREBPs intramembrane protease; MBTPS2; S2P
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O43462
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The transcription factors SREBPs (sterol regulatory element binding proteins) span the ER membrane and, in response to sterol depletion, the N-terminal domain of SREBPs are proteolytically activated, released from the membrane and then translocate to the nucleus where they induce the expression of genes regulating cholesterol metabolism. This proteolytic activation requires the sequential cleavage of SREBPs at Site-1, within the lumen of the ER, followed by cleavage at Site-2, within the first transmembrane domain. The cleavage at Site-1 separates the N-terminal and C-terminal domains of the protein and it requires the serine protease, S1P (Site-1 protease). Site-2 is subsequently processed by a putative zinc metalloprotease S2P, which releases the activated N-terminal domain for nuclear translocation. This proteolytic pathway is tightly regulated by sterol levels and is under the control of SCAP (SREBP cleavage-activating protein). SCAP, a sterol sensor, is latently bound to the C-terminal regulatory domains of the SREBPs, and it regulates cleavage of SREBPs at Site-1. Sterol levels influence the activity of SCAP, as SCAP is activated only in sterol-depleted cells and it inhibited by sterol accumulation.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: MBTPS2 polyclonal antibody detects endogenous levels of MBTPS2 protein.
Bioworld Molecular Weight: ~ 57 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of MBTPS2 polyclonal antibody at 1:500 dilution Lane 1: HEK293T whole cell lysate
Lane 2: RAW264.7 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human MBTPS2.
Conjugate: Unconjugated
Modification: Unmodified