Product Description

MBTPS2 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS61210-50, BS61210-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The transcription factors SREBPs (sterol regμlatory element binding proteins) span the ER membrane and, in response to sterol depletion, the N-terminal domain of SREBPs are proteolytically activated, released from the membrane and then translocate to the nucleus where they induce the expression of genes regμlating cholesterol metabolism. This proteolytic activation requires the sequential cleavage of SREBPs at Site-1, within the lumen of the ER, followed by cleavage at Site-2, within the first transmembrane domain. The cleavage at Site-1 separates the N-terminal and C-terminal domains of the protein and it requires the serine protease, S1P (Site-1 protease). Site-2 is subsequently processed by a putative zinc metalloprotease S2P, which releases the activated N-terminal domain for nuclear translocation. This proteolytic pathway is tightly regμlated by sterol levels and is under the control of SCAP (SREBP cleavage-activating protein). SCAP, a sterol sensor, is latently bound to the C-terminal regμlatory domains of the SREBPs, and it regμlates cleavage of SREBPs at Site-1. Sterol levels influence the activity of SCAP, as SCAP is activated only in sterol-depleted cells and it inhibited by sterol accumμlation.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB

Alternate Names: Membrane-bound transcription factor site-2 protease (EC:3.4.24.85), Endopeptidase S2P, Sterol regμlatory element-binding proteins intramembrane protease, SREBPs intramembrane protease, MBTPS2, S2P

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide corresponding to residues in Human MBTPS2.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MBTPS2 polyclonal antibody detects endogenous levels of MBTPS2 protein.

Molecular Weight: ~ 57 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O43462

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only