Product Description

Mcl-1 (S121) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1220-50, BS1220-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: B cell CLL/lymphoma-2 (Bcl-2) blocks cell death following a variety of stimμli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia-1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regμlator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase-3 (GSK-3) controls Mcl-1 stability, which has an effect on the regμlation of apoptosis by growth factors PI 3-kinase and Akt. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IP

Alternate Names: Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, MCL1, BCL2L3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human Mcl-1.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MCL1 (S121) polyclonal antibody detects endogenous levels of MCL1 protein.

Molecular Weight: ~ 37 kDa

Pathway: Regμlation of Apoptosis, Mitochondrial Control of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q07820

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only