MCM2 (2B5) Monoclonal Antibody-MB3156

MCM2 (2B5) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB3156-50, MB3156-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P49736

Host: Mouse

Reactivity: Human, Mouse

Applications: WB, IHC-F, IHC-P, ICC/IF, FC, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG1

Bioworld Molecular Weight: Calculated MW: 102 kDa; Observed MW: 125 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MCM2 in Jurkat, K562, 3T3 and Hela lysates using MCM2 antibody., Immunoprecipitation analysis of MCM2 in Hela lysates using MCM2 antibody., Immunofluorescence analysis of MCM2 in Hela cells using MCM2 antibody, and DAPI., Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MCM2 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28

Immunogen: Purified recombinant fragment of human MCM2 expressed in E. Coli.

Conjugate: Unconjugated

Modification: Unmodified