Product Description

MCM2 / BM28 (A11) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1221-50, BS1221-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regμlators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATPbinding motif, which is predicted to mediate ATP-dependent opening of doublestranded DNA. MCM proteins are regμlated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the ostreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, IP

Alternate Names: DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog Nuclear protein BM28, MCM2, BM28, CCNL1, CDCL1, KIAA0030

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human MCM2.

Dilution: WB: 1:500~1:1000
IHC/IF: 1:50~1:200
IP: 1:10~1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MCM2 (A11) polyclonal antibody detects endogenous levels of MCM2 protein.

Molecular Weight: ~ 125 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P49736

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only