Product Description

MCM5 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78652-50, BS78652-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P33992

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:1000 - 1:3000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200

Background: The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 100kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using MCM5 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MCM5 antibody. Western blot was performed from the immunoprecipitate using MCM5 antibody at a dilution of 1:1000.

Alternative Name: MCM5; CDC46; P1-CDC46

Immunogen: Recombinant fusion protein of human MCM5(NP_006730.2).

Conjugate: Unconjugated

Modification: Unmodified