Product Description

MCM6 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6661-50, BS6661-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14566

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC/IF: 1:50~1:200

Background: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MCM6 polyclonal antibody detects endogenous levels of MCM6 protein.

Bioworld Molecular Weight: ~ 110 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MCM6 polyclonal antibody, Immunohistochemistry (IHC) analysis of MCM6 polyclonal antibody in paraffin-embedded Human Lung tissue., Immunofluorescence analysis of U2OS cells, using MCM6 polyclonal antibody

Alternative Name: DNA replication licensing factor MCM6; MCG40308; Mcm6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog; S. pombe); MCM6 minichromosome maintenance deficient 6; MCM6; Minichromosome maintenance complex component 6; Minichromosome maintenance deficient (mis5 S. pombe) 6; Minichromosome maintenance deficient 6 homolog; Minichromosome maintenance protein 6; Mis5; MIS5 homolog; P105MCM;

Immunogen: Recombinant full length Human MCM6.

Conjugate: Unconjugated

Modification: Unmodified