Product Description

MCM8 Polyclonal Antibody

Catalogue Numbers: BS60306-50, BS60306-100

Sizes: 50µl, 100µl

Alternative Name: DNA helicase MCM8;
Minichromosome maintenance 8 ; MCM8; C20orf154

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UJA3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The mini-chromosome maintenance (MCM) family of proteins include MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5), MCM7 (Cdc47), MCM8, MCM9 and MCM10 (Dna43). The proteins in this family are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. MCM proteins are crucial components of the pre-replication complex (pre-RC) and are involved in replication fork formation and the recruitment of other DNA replication proteins. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP- dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. Throughout the cell cycle, MCM8 binds chromatin. MCM8 can interact with CDC6 and ORC2L.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MCM8 polyclonal antibody detects endogenous levels of MCM8protein.

Bioworld Molecular Weight: ~ 94 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MCM8 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human MCM8.

Conjugate: Unconjugated

Modification: Unmodified