Product Description

MCU Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90834-50, BS90834-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8NE86(Human) Q3UMR5(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex. Activity is regulated by MICU1 and MICU2. At low Ca2+ levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca2+ levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: MCU polyclonal antibody detects endogenous levels of MCU protein.

Bioworld Molecular Weight: Predicted band size 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Fig1:Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 3: mouse spleen tissue
Lane 2: HL-60
Lane 4: mouse brain tissue, ICC staining MCU in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HsMCU, MCU, C10orf42, CCDC109A, C109A, C10orf42, Calcium uniporter protein mitochondrial, Ccdc109a, Coiled-coil domain-containing protein 109A, HsMCU, Mitochondrial Calcium Uniporter,

Immunogen: Recombinant protein within human MCU aa 50-250.

Conjugate: Unconjugated

Modification: Unmodified