MDFI Polyclonal Antibody-BS75422

MDFI Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS75422-50, BS75422-100

Swiss-Prot: Q99750

Host: Rabbit

MDFI Polyclonal Antibody-BS75422

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 35kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using MDFI antibody at 1:3000 dilution., Immunofluorescence analysis of U-2 OS cells using MDFI Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: MDFI; I-MF; I-mfa

Immunogen: Recombinant fusion protein of human MDFI(NP_005577.1).

Modification: Unmodified

Conjugate: Unconjugated