Product Description

MDK (D143) Polyclonal Antibody

Catalogue Numbers: BS3568-50, BS3568-100

Sizes: 50µl, 100µl

Alternative Name: Midkine; MK; Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; MDK; MK1; NEGF2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P21741

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Midkine, or MK, is a heparin-binding molecule involved in the regulation of growth and differentiation during embryogenesis. MK expression is tightly regulated during embryonic development by steroid receptors of the retinoic acid superfamily. The mature human MK protein is 118 amino acids in length and contains five intrachain disulfide bonds. MK is a non-glycosylated protein that shows greater than 87% identity between human and mouse. The carboxy-terminus of MK contains the principle heparin-binding site and the molecule’s neurite-promoting sequences; both the amino- and carboxy-terminal sequences are required for the molecule’s neurotrophic properties.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MDK (D143) polyclonal antibody detects endogenous levels of MDK protein.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MDK (D143) pAb at 1:500 dilution
Lane 1: A375 whole cell lysate(40ug)
Lane 2: HCT116 whole cell lysate(40ug)
Lane 3: CT26 whole cell lysate(40ug)
Lane 4: The Testis tissue lysate of Rat(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 120-170 of Human MDK.

Conjugate: Unconjugated

Modification: Unmodified