MDK Polyclonal Antibody-BS6038

MDK Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6038-50, BS6038-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P21741

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 16KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of SH-SY5Y cells, using MDK antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s., Immunofluorescence analysis of HeLa cells using MDK Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using MDK Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining.

Alternative Name: MDK; ARAP; MK; NEGF2; midkine

Immunogen: Recombinant fusion protein of human MDK(NP_002382.1).

Conjugate: Unconjugated

Modification: Unmodified