Product Description

MDM1 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65825-50, BS65825-100

Swiss-Prot: Q8TC05

Host: Rabbit

MDM1 Polyclonal Antibody-BS65825

Storage and Stability: Store at 4°C short term. Aliquot and store at -2°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Pig, Horse,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: This gene encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles, is amplified in the mouse transformed 3T3 cell line, and the encoded protein is able to bind to p53. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Purification and Purity: affinity purified by Protein A

Specificity: MDM1 Polyclonal Antibody detects endogenous levels of MDM1 protein.

Bioworld Molecular Weight: ~81 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (Rat brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MDM1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (Mouse brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MDM1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Alternative Name: FLJ95264; MDM 1; Mdm1; Mdm1 nuclear protein homolog (mouse); Mdm1 nuclear protein homolog; MDM1_HUMAN; Mdm4 transformed 3T3 cell double minute 1 p53 binding protein (mouse); Mdm4 transformed 3T3 cell double minute 1 p53 binding protein; Mouse Mdm1 nuclear protein homolog; Nuclear protein double minute 1; Nuclear protein MDM1.

Immunogen: KLH conjugated synthetic peptide derived from human MDM1:81-180/714

Modification: Unmodified

Conjugate: Unconjugated