Product Description

ME3 (R576) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3339-50, BS3339-100

Sizes: 50µl, 100µl

Swiss-Prot: Q16798

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: ME3 (malic enzyme 3), also known as NADP-ME (NADP-dependent malic enzyme, mitochondrial), is a 604 amino acid protein of the mitochondrial matrix that catalyzes the conversion of (S)-malate and NADP+ to pyruvate, carbon dioxide and NADPH. A member of the malic enzymes family, ME3 utilizes manganese and magnesium as cofactors and is expressed in tissues that divide at a low rate. The gene encoding ME3 maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ME3 (R576) polyclonal antibody detects endogenous levels of ME3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ME3 (R576) pAb at 1:500 dilution
Lane 1: Panc1 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: The Testis tissue lysate of Mouse (10ug), Immunohistochemistry (IHC) analyzes of ME3 (R576) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 67 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: NADP-dependent malic enzyme, mitochondrial; NADP-ME; Malic enzyme 3; ME3

Immunogen: Synthetic peptide, corresponding to amino acids 547-598 of Human ME3.

Conjugate: Unconjugated

Modification: Unmodified