MED12 Polyclonal Antibody
Catalogue Numbers: BS61060-50, BS61060-100
Sizes: 50µl, 100µl
Alternative Name: Mediator of RNA polymerase II transcription subunit 12; ARC240; CAG repeat protein 45; Mediator complex subunit 12; OPA-containing protein; Trap230; Trinucleotide repeat-containing gene 11 protein; MED12
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q93074
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, that are characterized by the presence of a nuclear receptor recognition motif which mediates the ligand-dependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: MED12 polyclonal antibody detects endogenous levels of MED12 protein.
Bioworld Molecular Weight: ~ 243 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of MED12 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: H9C2 whole cell lysate
Lane 3: sp20 whole cell lysate
Lane 4: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human MED12.
Conjugate: Unconjugated
Modification: Unmodified