Product Description

MED15 Polyclonal Antibody

Catalogue Numbers: BS60172-50, BS60172-100

Sizes: 50µl, 100µl

Alternative Name: Mediator of RNA polymerase II transcription subunit 15; Activator-recruited cofactor 105 kDa component; ARC105; CTG repeat protein 7a; Mediator complex subunit 15; Positive cofactor 2 glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; TPA-inducible gene 1 protein; TIG-1; Trinucleotide repeat-containing gene 7 protein; ARC105; CTG7A; PCQAP; TIG1; TNRC7

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96RN5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The Mediator complex is one such multiprotein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med15 (Mediator of RNA polymerase II transcription subunit 15), also known as ARC105, CTG7A, PCQAP, TIG1 or TNRC7, is a 788 amino acid subunit of the Mediator complex that localizes to both the nucleus and the cytoplasm. Expressed ubiquitously with highest expression in placenta and blood, Med15 participates in the regulation of Pol II-mediated gene expression and is thought to play a key role in the control of lipid homeostatsis. The gene encoding Med15 is located in a region on chromosome 22 that is deleted in DiGeorge syndrome, suggesting that the loss of Med15 may be associated with this rare congenital disease. Due to alternative splicing events, Med15 is expressed as two isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MED15 polyclonal antibody detects endogenous levels of MED15 protein.

Bioworld Molecular Weight: ~ 87 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MED15 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human MED15.

Conjugate: Unconjugated

Modification: Unmodified