Product Description

MED18 (V102) Polyclonal Antibody

Catalogue Numbers: BS3141-50, BS3141-100

Sizes: 50µl, 100µl

Alternative Name: Mediator of RNA polymerase II transcription subunit 18; Mediator complex subunit 18; p28b; MED18

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BUE0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med18 (mediator complex subunit 18), also known asp28b, is a 208 amino acid protein that localizes to nucleus and exists as a component of the mediator complex. Working in tandem with several other proteins, including Med8 and Med25, Med18 serves as a scaffold for the assembly of a functional preinitiation complex with Pol II and general transcription factors, thereby activating the transcription of Pol II-dependent genes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MED18 (V102) polyclonal antibody detects endogenous levels of MED18 protein.

Bioworld Molecular Weight: ~ 24 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MED18 (V102) pAb at 1:1000 dilution
Lane 1: HepG2 whole cell lysate(20ug)
Lane 2: SGC7901 whole cell lysate(20ug)
Lane 3: The Brain tissue lysate of Rat(40ug)
Lane 4: The Brain tissue lysate of Mouse(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 72-120 of Human MED18.

Conjugate: Unconjugated

Modification: Unmodified