MED22 Polyclonal Antibody-BS60173

MED22 Polyclonal Antibody

Catalogue Numbers: BS60173-50, BS60173-100

Sizes: 50µl, 100µl

Alternative Name: Mediator of RNA polymerase II transcription subunit 22; Mediator complex subunit 22; Surfeit locus protein 5; Surf-5; MED22; SURF5

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15528

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (Mediator complex subunit 22), also known as SURF5 (Surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MED22 polyclonal antibody detects endogenous levels of MED22 protein.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MED22 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Rat liver tissue lysate

Immunogen: A synthetic peptide corresponding to residues in Human MED22.

Conjugate: Unconjugated

Modification: Unmodified