MED28 Polyclonal Antibody
Catalogue Numbers: BS60525-50, BS60525-100
Sizes: 50µl, 100µl
Alternative Name: Mediator of RNA polymerase II transcription subunit 28; Endothelial-derived protein 1; Mediator complex subunit 28; Merlin and Grb2-interacting cytoskeletal protein; Magicin; Tumor angiogenesis marker EG-1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9H204
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The Mediator complex is one such multiprotein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med28 (mediator complex subunit 28), also known as EG1 (endothelial-derived protein 1), magicin (merlin and Grb2-interacting cytoskeletal protein) or tumor angiogenesis marker EG-1, is a 178 amino acid subunit of the Mediator complex that localizes to both nucleus and cytoplasm. Widely expressed with highest expression in vascular tissues such as placenta, testis and liver, Med28 participates in the regulation of Pol II-mediated gene expression and is thought to play a key role in signal regulation and cell proliferation. It is suggested that Med28 functions as a repressor of SMC (smooth muscle cell) differentiation and may be associated with abnormalities in SMC growth and differentiation, including atherosclerosis, asthma, hypertension and smooth muscle tumors.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: MED28 polyclonal antibody detects endogenous levels of MED28 protein.
Bioworld Molecular Weight: ~ 20 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of MED28 polyclonal antibody at 1:500 dilution
Lane 1: HEK293Twhole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human MED28
Conjugate: Unconjugated
Modification: Unmodified