Product Description

MED29 Polyclonal Antibody

Catalogue Numbers: BS60174-50, BS60174-100

Sizes: 50µl, 100µl

Alternative Name: Mediator of RNA polymerase II transcription subunit 29; Intersex-like protein; Mediator complex subunit 29; MED29; IXL

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NX70

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med29 (mediator complex subunit 29), also known as IXL (intersex-like), is a 200 amino acid nuclear protein and component of the mediator complex. Widely expressed in embryo and adult tissue, Med29 is considered a novel amplification target gene in pancreatic cancer and is encoded by a gene that maps to human chromosome 19q13.2. Chromosome 19 consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MED29 polyclonal antibody detects endogenous levels of MED29 protein.

Bioworld Molecular Weight: ~ 24 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MED29 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat lung tissue lysate

Immunogen: A synthetic peptide corresponding to residues in Human MED29.

Conjugate: Unconjugated

Modification: Unmodified