Product Description

MED30 Polyclonal Antibody

Catalogue Numbers: BS60175-50, BS60175-100

Sizes: 50µl, 100µl

Alternative Name: Mediator of RNA polymerase II transcription subunit 30; Mediator complex subunit 30; TRAP/Mediator complex component TRAP25; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; Trap25; THRAP6; TRAP25

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96HR3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Med30 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. The mediator complex functions as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, and is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Found in the nucleus, Med30 is expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MED30 polyclonal antibody detects endogenous levels of MED30 protein.

Bioworld Molecular Weight: ~ 19 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MED30 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human MED30.

Conjugate: Unconjugated

Modification: Unmodified