Product Description

MED31 Polyclonal Antibody

Catalogue Numbers: BS60506-50, BS60506-100

Sizes: 50µl, 100µl

Alternative Name: Mediator of RNA polymerase II transcription subunit 31; Mediator complex subunit 31; Mediator complex subunit SOH1; hSOH1; MED31; SOH1; CGI-125

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y3C7

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Med31 (mediator of RNA polymerase II transcription subunit 31), also known as Soh1 or CGI-125, is a 131 amino acid protein that belongs to the mediator complex subunit 31 family. Mediator proteins serve as connectors between transcriptional activators and basal transcription machinery. Med31 is an evolutionary conserved coregulator of RNA polymerase II transcription and is a stable component of the Mediator complex. It is involved in forming a scaffold with other regulatory proteins for the assembly of a functional preinitiation complex with RNA polymerase II and general transcription factors. Med31 is required for normal levels of gene conversion during meiosis and contributes in the coordination of cellular processes by regulating the expression of larger sets of genes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MED31 polyclonal antibody detects endogenous levels of MED31 protein.

Bioworld Molecular Weight: ~ 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MED31 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: MCF-7 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human MED31.

Conjugate: Unconjugated

Modification: Unmodified