Product Description

MED6 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71111-50, BS71111-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75586

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using MED6 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.

Bioworld Molecular Weight: 28kDa

Specificity: Polyclonal Antibodies

Alternative Name: MED6; ARC33; NY-REN-28

Immunogen: Recombinant fusion protein of human MED6(NP_005457.2).

Conjugate: Unconjugated

Modification: Unmodified