Product Description

MEK-4/ MAP2K4 (phospho-T261) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4168-50, BS4168-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regμlatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38β. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4, MAPKK 4, JNK-activating kinase 1, MAPK/ERK kinase 4, MEK 4, SAPK/ERK kinase 1, SEK1, Stress-activated protein kinase kinase 1, SAPK kinase 1, SAPKK-1, SAPKK1, c-Jun N-terminal kinase kinase 1, JNKK, MAP2K4, JNKK1, MEK4, MKK4, , PRKMK4, SEK1, SERK1, SKK1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human MEK-4 around the phosphorylation site of Threonine 261.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-SEK1/MKK4 (T261) polyclonal antibody detects endogenous levels of SEK1/MKK4 protein only when phosphorylated at Thr261

Molecular Weight: ~ 44 kDa

Pathway: SAPK JNK Signaling Cascades, Signaling Pathways Activating p38 MAP KinaseMAPK Erk in Growth and Differentiation Pathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P45985

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only