MEK1/2 Monoclonal Antibody-MB11205

MEK1/2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11205-50, MB11205-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q02750/P36507

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 43,44 kDa; Observed MW: 43,44 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MEK1/2 in K562, C6, 3T3, Hela lysates using MEK1/2 antibody., Immunocytochemistry analysis of MEK1/2 in Hela using MEK1/2 antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human colon cancer using MEK1/2 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein k

Immunogen: Recombinant protein of human MEK1/2

Conjugate: Unconjugated

Modification: Unmodified