MEK1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90838-50, BS90838-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q02750(Human) P31938(Mouse) Q01986(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat, Cow, Dog
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MEK1 polyclonal antibody detects endogenous levels of MEK1 protein.
Bioworld Molecular Weight: 43 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MEK1 on different lysates using anti-MEK1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: A431
Lane 2: HepG2
Lane 3: Hela, ICC staining MEK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MAP kinase kinase 1, MAPKK 1, MKK1, MEK 1, MAP2K1, Dual specificity mitogen activated protein kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP kinase/Erk kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, Mek1, MEKK1, Mitogen activated protein kinase kinase 1, MKK 1, MKK1, MP2K1, PRKMK1, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1), Protein kinase mitogen activated, kinase 1,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified