MEK2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90839-50, BS90839-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P36507(Human) Q63932(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:50-1:100
Background: A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MEK2 polyclonal antibody detects endogenous levels of MEK2 protein.
Bioworld Molecular Weight: 44 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MEK2 on different lysates using anti-MEK2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: 293T, ICC staining MEK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MAP kinase kinase 2, MAPKK 2, MEK 2, MAP2K2, Cardiofaciocutaneous syndrome, CFC syndrome, CFC4, Dual specificity mitogen activated protein kinase kinase 2, Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, FLJ26075, MAP kinase kinase 2, map2k2, MAPK / ERK kinase 2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, Microtubule associated protein kinase kinase 2, Mitogen activated protein kinase kinase 2, Mitogen activated protein kinase kinase 2 p45, MKK 2, MKK2, MP2K2, OTTHUMP00000165826, OTTHUMP00000165827, PRKMK 2, PRKMK2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified