MEK3/6 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90841-50, BS90841-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P46734(Human) P52564(Human) O09110(Mouse) P70236(Mouse) EntrezGene:114495(Rat) EntrezGene:303200(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MEK3/6 polyclonal antibody detects endogenous levels of MEK3/6 protein.
Bioworld Molecular Weight: 39/37 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MEK3+MEK6 on different lysates using anti-MEK3+MEK6 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat, ICC staining MEK3+MEK6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MAP kinase kinase 3, MAPKK 3, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2, MAP2K3, MAP kinase kinase 6, MAPKK 6, MEK 6, SAPK kinase 3, SAPKK-3, SAPKK3, MAP2K6, Dual specificity mitogen activated protein kinase kinase 6, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAP kinase kinase 6, map2k3, MAP2K6, MAPK/ERK kinase 3, MAPK/ERK kinase 6, MAPKK 3, MAPKK 6, MAPKK3, MAPKK6, MEK 3, MEK 6, Mitogen activated protein kinase kinase 3, Mitogen activated protein kinase kinase 6, MKK3, MKK6, MP2K3, PRKMK3, PRKMK6, Protein kinase, mitogen activated, kinase 6 (MAP kinase kinase 6), SAPK kinase 2, SAPKK 3, SAPKK-2, SAPKK2, SAPKK3, Stress activated protein kinase kinase 3, Stress-activated protein kinase kinase 2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified