MEK5 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90842-50, BS90842-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13163(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
Background: A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MEK5 polyclonal antibody detects endogenous levels of MEK5 protein.
Bioworld Molecular Weight: 49 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MEK5 on Hela cells lysates using anti-MEK5 antibody at 1/1,000 dilution., ICC staining MEK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MAP kinase kinase 5, MAPKK 5, MEK 5, MAP2K5, Dual specificity mitogen activated protein kinase kinase 5, Dual specificity mitogen-activated protein kinase kinase 5, EC 2.7.12.2, HsT17454, MAP kinase kinase 5, MAP kinase kinase MEK5b, MAP2K5, MAPK/ERK kinase 5, MAPKK 5, MAPKK5, MEK 5, mitogen-activated protein kinase kinase 5, MKK5, MP2K5, PRKMK5, Protein kinase, mitogen-activated, kinase 5, SAPKK5, SKK5,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified