MEKK3 Polyclonal Antibody-BS90845

MEKK3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90845-50, BS90845-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99759(Human) Q61084(Mouse) Unigene:72680(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: MAP kinase kinase kinase (MEKK3 or MAP3K3) is a serine/threonine protein kinase that activates SAPK and ERK via phosphorylation and activation of their respective MAP kinase kinases, SEK and MEK1/2. MEKK3 also stimulates MEK5 via activation of ERK5/BMK1, which is at least partly regulated by a direct interaction between MEK5 and MEKK3 via p67phox-Bem1p (PB1) protein-protein interaction domains found in both proteins. MEKK3 modulates NF-κB activation in response to a variety of agonists including TNFα, LPS, IL-1 and LPA. Despite reports showing that phosphorylation of MEKK3 at Ser526 within the activation loop is necessary for kinase activation, at least one study suggests that dual phosphorylation at Thr516 and Ser520 is required for LPA-stimulated IKKβ/NF-κB activation. Phosphorylation at Thr294 appears to negatively regulate MEKK3 by promoting 14-3-3β binding and inhibition of the kinase activity.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: MEKK3 polyclonal antibody detects endogenous levels of MEKK3 protein.

Bioworld Molecular Weight: 71 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: A431, ICC staining MEKK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MEK kinase 3, MEKK 3, MAP3K3, M3K3, MAP/ERK kinase kinase 3, MAP3K3, MAPK/ERK kinase kinase 3, MAPKKK3, MEK kinase 3, MEKK 3, MEKK3, Mitogen activated protein kinase kinase kinase 3, Mitogen-activated protein kinase kinase kinase 3,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified