Product Description

MELK (N449) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2309-50, BS2309-100

Sizes: 50µl, 100µl

Swiss-Prot: Q14680

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC, IF

Background: Maternal embryonic leucine zipper kinase (KIAA0175, HPK38) or MELK, a new member of the Snf1/AMPK family of kinases, encodes a protein with a kinase catalytic domain and a leucine zipper motif consisting of a periodic repetition of leucine residues at every seventh residue located within the N-terminal catalytic domain. This motif has been observed in myriad DNA-binding proteins and is presumed to be involved in protein-DNA interactions, and potentially protein-protein interactions. Research predicts that the gene product of MELK plays a role in the signal transduction events in the egg and early embryo. Mouse and human MELK proteins share 95% sequence identity in the kinase domain and northern blot analysis in mouse indicates that MELK expression is restricted to spermatogonia in the testis and to oocytes in the ovary.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MELK (N449) polyclonal antibody detects endogenous levels of MELK protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MELK (N449) pAb at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: SK-OVCAR3 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (20ug)
Lane 4: Hela whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of MELK (N449) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 75 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Maternal embryonic leucine zipper kinase; hMELK; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK; MELK; KIAA0175; Pk38; mPK38

Immunogen: Synthetic peptide, corresponding to amino acids 415-473 of Human MELK.

Conjugate: Unconjugated

Modification: Unmodified