MEP50 Monoclonal Antibody-MB0172

MEP50 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0172-50, MB0172-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BQA1

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, ICC

All Applications: WB: 1:1000
ICC: 1:50~200

Background: WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061).This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of MEP50 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 42KDa
Observed band size: 42KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of MEP50 antibody in C6, 3T3 and K562 cell lysates using MEP50 antibody (1:1000 diluted)., Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100,next using anti-MEP50 antibody (dilution 1:100).

Alternative Name: Methylosome protein 50; MEP-50; Androgen receptor cofactor p44; WD repeat-containing protein 77; p44/Mep50; WDR77; MEP50; WD45; HKMT1069; Nbla10071

Immunogen: Recombinant full length Human MEP50.

Conjugate: Unconjugated

Modification: Unmodified