Mesothelin Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90847-50, BS90847-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13421 Human
Host: Rabbit
Reactivity: Human
Applications: WB, ICC IHC
All Applications: WB:1:500-1:2,000
ICC:1:50
IHC:1:50-1:200
Background: This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. Mesothelin is over expressed in several human tumors, including mesothelioma, ovarian cancer, pancreatic adenocarcinoma and cholangiocarcinoma. The interaction between mesothelin and MUC16 (also known as CA125) may facilitate the implantation and peritoneal spread of tumors by cell adhesion. Dr. Mitchell Ho and colleagues at the National Cancer Institute identified the region (residues 296-359) consisting of 64 amino acids at the N-terminus of cell surface mesothelin as the functional binding domain for MUC16.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Specificity: Mesothelin polyclonal antibody detects endogenous levels of Mesothelin protein.
Bioworld Molecular Weight: 45 kDa, cleavage form
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Mesothelin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: Hela cell lysate, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ER1803-76 at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Alternative Name: MPF, MSLN, MPF, CAK 1, CAK1, CAK1 antigen, cleaved form, Megakaryocyte potentiating factor, Mesothelin, Mesothelin isoform 1 precursor, MPF, Msln, MSLN, Pre pro megakaryocyte potentiating factor, Pre-pro-megakaryocyte-potentiating factor, SMR, SMRP, Soluble MPF mesothelin related protein antibody
Immunogen: Recombinant protein within Human Mesothelin aa 220-410.
Conjugate: Unconjugated
Modification: Unmodified