Product Description

Methyl-HP1 gamma/CBX3-H175 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74117-50, BS74117-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13185

Host: Rabbit

Reactivity: Human

Applications: IHC

All Applications: IHC,1:50 - 1:200

Background: At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Methylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] HP1 gamma/HP1 gamma/CBX3 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] HP1 gamma/HP1 gamma/CBX3 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] HP1 gamma/HP1 gamma/CBX3 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: CBX3; HECH; HP1-GAMMA; HP1Hs-gamma; chromobox 3

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human HP1 gamma/CBX3(NP_009207.2).

Conjugate: Unconjugated

Modification: Methylated