METTL14 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-93402-60, E-AB-93402-120, E-AB-93402-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: METTL14
Target Synonym: METTL14; hMETTL14
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC, IF, IP
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q9HCE5
Background: The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A, which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity. In embryonic stem cells (ESCs, m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity. M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human METTL14
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200, IP 1:50-1:200
Calculated MW: 52kDa
Observed MW: 65KDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only