MGEA5 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90850-50, BS90850-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O60502(Human) Q9EQQ9(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, IHC, IP,
All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
IP:1:10-1:50
Background: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: MGEA5 polyclonal antibody detects endogenous levels of MGEA5 protein.
Bioworld Molecular Weight: 103 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution., Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.
Alternative Name: OGA, NCOAT, MGEA5, HEXC, KIAA0679, MEA5, Beta-hexosaminidase, Beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, Bifunctional protein NCOAT, HEXC, Hyaluronidase in meningioma, KIAA0679, MEA5, Meningioma expressed antigen 5, Meningioma-expressed antigen 5, MGEA 5, Mgea5, N-acetyl-beta-D-glucosaminidase, N-acetyl-beta-glucosaminidase, NCOAT, Nuclear cytoplasmic O GlcNAcase and acetyltransferase, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, OGA, OGA, Protein O-GlcNAcase,
Immunogen: Recombinant protein of N-terminal human MGEA5.
Conjugate: Unconjugated
Modification: Unmodified