Product Description

MGMT (C5) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2540-50, BS2540-100

Sizes: 50µl, 100µl

Swiss-Prot: P16455

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, in a study of oral cancer cell lines, high MGMT activity was associated with the presence of mutant p53. Similarly, MGMT activity was significantly lower in ovarian tumors with wild-type p53 than in tumors with mutant p53, supporting the view that wild type p53 down-regulates the basal MGMT promoter.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MGMT (C5) polyclonal antibody detects endogenous levels of MGMT protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MGMT (C5) pAb at 1:500 dilution
Lane 1: L02 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: PC3 whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug)
Lane 5: PC12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of MGMT (C5) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 22 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: MGMT; EC 2.1.1.63; Methylated-DNA--protein-cysteine methyltransferase

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human MGMT.

Conjugate: Unconjugated

Modification: Unmodified