MGMT Polyclonal Antibody-BS90853

MGMT Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90853-50, BS90853-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16455

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
ICC:1:200
IHC:1:200
FC:1:100

Background: MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O (6) position of guanine, restoring guanine to its normal form without causing DNA breaks. MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents. It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: MGMT polyclonal antibody detects endogenous levels of MGMT protein.

Bioworld Molecular Weight: 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7
Lane 3: Daudi
Lane 4: Raji
Lane 5: SK-BR-3
Lane 6: SKOV3
Lane 7: Human liver
Lane 8: Human kidney
Lane 9: Human thymus
Lane 10: Human placentae, ICC staining MGMT in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MGMT, 6 O methylguanine DNA methyltransferase, 6-O-methylguanine-DNA methyltransferase, Agat, AGT, AI267024, EC 2.1.1.63, Methylated DNA protein cysteine methyltransferase, Methylated-DNAprotein-cysteine methyltransferase, Methylguanine DNA methyltransferase, MGC107020, MGMT, MGMT, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified