Product Description

MGST1 (R74) Polyclonal Antibody

Catalogue Numbers: BS3879-50, BS3879-100

Sizes: 50µl, 100µl

Alternative Name: Microsomal glutathione S-transferase 1; Microsomal GST-1; Microsomal GST-I; MGST1; GST12; MGST

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10620

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Microsomal glutathione transferase 1 (MGST1) is an abundant protein that catalyzes the conjugation of electrophilic compounds with glutathione, as well as the reduction of lipid hydroperoxides. The level of MGST1 mRNA in rat are highest in liver, while the levels of expression vary in non-hepatic tissues. In addition, the subcellular localization patterns of MGST1 are highly tissue specific. In hepatocytes and bile ducts, MGST1 is localized to the cytoplasm. MGST1 is localized to the nucleus in choroid plexus and primary spermatocytes and displays a granular cytoplasmic staining pattern in the adrenal medulla). Also, focal staining of MGST1 is observed in the distal tubules and collecting ducts of the liver.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MGST1 (R74) polyclonal antibody detects endogenous levels of MGST1 protein.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MGST1 (R74) pAb at 1:1000 dilution
Lane 1: HepG2 whole cell lysate(20ug)
Lane 2: HCC827 whole cell lysate(10ug)
Lane 3: The Liver tissue lysate of Rat(40ug)
Lane 4: The Liver tissue lysate of Mouse(40ug)
Lane 5: 3T3-L1 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 41-90 of Human MGST1.

Conjugate: Unconjugated

Modification: Unmodified