MHC class I Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90855-50, BS90855-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P30443(Human) P01889(Human) P01891(Human) P01892(Human) P13746(Human) P16188(Human) P30450(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MHC class I polyclonal antibody detects endogenous levels of MHC class I protein.
Bioworld Molecular Weight: 41 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MHC class I on different lysates using anti-MHC class I antibody at 1/1,000 dilution.
Positive control:
Lane 1: Raji
Lane 2: THP-1, ICC staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HLA-A, HLA-B, A 28, A 9, Antigen presenting molecule, Aw 24, Aw 68, CLASS I HISTOCOMPATIBILITY ANTIGEN, H2 K1, H2K, HLA A, HLA class I histocompatibility antigen A 1 alpha chain, HLA class I histocompatibility antigen A 11 alpha chain, HLA class I histocompatibility antigen A 2 alpha chain, HLA class I histocompatibility antigen A 24 alpha chain, HLA class I histocompatibility antigen A 3 alpha chain, HLA class I histocompatibility antigen A 30 alpha chain, HLA class I histocompatibility antigen A 32 alpha chain, HLA class I histocompatibility antigen A 68 alpha chain, HLAA, Leukocyte antigen class I A, Major histocompatibility complex class I A, MHC class I antigen A 1, MHC class I antigen A*11, MHC class I antigen A*2, MHC class I antigen A*24, MHC class I antigen A*3, MHC class I antigen A*30, MHC class I antigen A*32, MHC class I antigen A*68, MHC class I antigen HLA A heavy chain, MHC class I heavy chain H2 K,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified